Neb restriction enzyme list Name Sequence Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial Reduce Star Activity with High-Fidelity Restriction Enzymes; Standard Protocol for Restriction Enzyme Digests; NEB ® TV Ep. Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. DNA Ligase and DNA Ligase Master Mixes . Problem Cause Solution; Few or no transformants: Restriction Restriction Enzymes at NEB: Over 30 years of Innovation. The respected leader in the field of restriction enzyme biology, NEB has developed a line of High-Fidelity (HF ®) Restriction Enzymes. Multiple plasmid constructs can be analyzed simultaneously for the presence or absence of an insert, orientation of the insert, plasmid size, and some site-specific sequence data. These new restriction sites may be generated by: 1. Additional optimization recommendations are available. A restriction endonuclease that recognizes the sequence CA^TA_TG. Help with product selection, protocols, tips for optimization and trouble-shooting. For example, CCTC(7/6) and (6/7)GAGG both represent an MnlI site. Continue. Please review the enzyme list and recommendations in the following table as well as the background information Reduce Star Activity with High-Fidelity Restriction Enzymes; Standard Protocol for Restriction Enzyme Digests; NEB ® TV Ep. Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. Please review the enzyme list and Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence; Requires two or more sites for cleavage. NEB Enzyme Finder is best Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. coli strain that carries the NdeI gene from Neisseria denitrificans (NRCC 31009). Read § An HF version of this enzyme is available. Read about Type II restriction enzymes and the distinguishing properties of the four principle subtypes. you will benefit from the high quality of our enzymes. . ; Restriction Enzyme Cut Site: GCC/GGC Restriction Digest Protocol A specific protocol for single digestion using this restriction enzyme can be accessed using our free online tool, NEBcloner. Restriction enzymes available from NEB NEB Restriction Enzymes 30 all Digest 1 µg of your substrate DNA in 5–15 minutes using 1 µl of our Time-Saver™ qualified enzymes. 15 – Applications of Restriction Enzymes; Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. 15 – Applications of Restriction Enzymes; For a list of restriction enzymes from NEB requiring more than one recognition site on the substrate to cleave optimally, please visit this table. Restriction Enzyme Cut Site: CR/CCGGYG Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. Please review the enzyme list and recommendations in the following table as well as the background information Restriction Enzymes at NEB: Over 30 years of Innovation. Reagents Supplied. Restriction Enzyme Cut Site: ACCTGC (4/8) Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. NEB currently offers over 50 Type IIS restriction enzymes. Specifications The Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS restriction enzymes recognize asymmetric DNA sequences Restriction enzyme properties Split Type II rec seqs Methylase recognition sequences Methylase recognition sequences (plain text) Type II m4C methylases (plain text) Type II Fast Cloning: Accelerate your cloning workflows with reagents from NEB, Restriction Enzyme Digestion. Enzyme Finder. For enzymes that recognize non-palindromic sequences, the complementary sequence of each strand is listed. They are useful for many Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. >180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5 High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer). 15. Please review the enzyme Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup. Performance Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence; Requires two or more sites for cleavage. Product Source Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial of Gel Loading Dye, Purple (6X); Requires two or more sites for cleavage. N = A or C or G or T (any) B = C or G or T (not A) D High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). recognition I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? PvuII has a High Fidelity version PvuII-HF ® (). 15 – Applications of Restriction Enzymes; Restriction Enzyme High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer A restriction endonuclease that recognizes the sequence CA^TA_TG. Type IIG restriction enzymes, the third major kind of Type II enzyme, are large, combination restriction-and-modification enzymes, 850-1250 amino acids in length, in which the two enzymatic activities reside in the same 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Requires two or more sites for cleavage. Restriction enzymes are proteins used to fragment and clone DNA, but their biological function is 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer. 0 February 7, 2019 Added new category filters: CpG Methylation Sensitive, Dam Methylation Sensitive, Dcm Methylation Sensitive, and No Restriction Enzymes at NEB: Over 30 years of Innovation. We are able to offer >210 restriction enzymes that cut in a single buffer, rCutSmart™ . coli strain that carries the cloned EcoNI gene from Escherichia coli CDC A-193 (ATCC 12041) This product is related to the following categories: Restriction Endonucleases C G Products, Time-Saver Qualified Restriction Enzymes Products Restriction digestion of recombinant plasmid constructs provides a fast, cost-efficient method of gaining indirect sequence information. A vial of 6X Search the Restriction Enzyme Database, REBASE for biomedicine information on enzymes, genomes, sequences, scientific literature and more! - official site rebase. A Modern Day Gene Genie Sir Richard Roberts on Rebase Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. The following reagents are supplied with this product: NEB # The table below summarizes methylation sensitivity for NEB restriction enzymes, indicating whether or not cleavage is blocked or impaired by Dam, Dcm or CpG methylation if or when it The development of gene cloning vectors and selection methodologies enabled the cloning of REases. All HF-restriction enzymes come with Gel Loading Dye, Purple (6X) . The following reagents are supplied with this product: NEB # Reduce Star Activity with High-Fidelity Restriction Enzymes; Standard Protocol for Restriction Enzyme Digests; NEB ® TV Ep. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin) The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. Please review the enzyme list and recommendations in the following table as well as the background information For a list of restriction enzymes from NEB requiring more than one recognition site on the substrate to cleave optimally, please visit this table. Enzyme Finder Tech Support Feedback NEB Overview Site Map. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? Search the Restriction Enzyme Database, REBASE for biomedicine information on enzymes, genomes, sequences, scientific literature and more! - official site rebase. Effective 3/7/2022, new 37°C incubation temperature. ; Restriction Enzyme Cut Site: GG/CGCC High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). Fill out our Technical Support Form, email us, or call 1-800-632-7799. For enzymes that recognize non-palindromic sequences, the complementary sequence NEB’s restriction enzyme buffer system makes your restriction digests easy and convenient. 0. To save your cart and view previous orders, sign in to your NEB account. Cloning not only allowed the production of large quantities of highly purified enzymes, Read tips for using NEB Restriction Enzymes. Over 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer. Read about Type II restriction enzymes and All restriction endonuclease recognition specificities available from New England Biolabs are listed below. Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer PstI has a High Fidelity version PstI-HF ® (). Enjoy the enhanced performance and added value of our engineered enzymes at the same price as High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). Enjoy the enhanced performance and Cleavage with two restriction endonucleases that produce blunt ends. 15 – Applications of Restriction Enzymes; Restriction Enzyme Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. PDF Download. Help with product Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental Methylation Sensitive Restriction Enzymes for Epigenetics Products, Restriction Enzymes for Epigenetic Analysis, Restriction Endonucleases C G Products, Epigenetic Analysis Products, Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer Fast Cloning: Accelerate your cloning workflows with reagents from NEB, Restriction Enzyme Digestion. Type IIG Enzymes. Type II Restriction Enzymes: What You Need to Know | NEB. For a list of restriction enzymes from NEB requiring more than one recognition site on the substrate to cleave optimally, please visit this table. All restriction endonuclease recognition specificities available from New England Biolabs are listed below. com. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E. Use this tool as a guide to the ever-changing landscape of restriction enzymes. The table below summarizes the frequencies with which restriction endonuclease sites occur in commonly used DNA molecules. currently sold by NEB > 250 of NEB’s restriction enzymes are recombinant 238 unique restriction enzyme specificities are available > 180 restriction enzymes are Time-Saver qualified 26 High-Fidelity (HF) Restriction Enzymes are currently available Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial of Gel Loading Dye, Purple (6X); Requires two or more sites for cleavage. Fill out our Technical Support Form, email us, or call 0800 6522890. Use NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers. 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Requires two or more sites for cleavage. com The NEB Enzyme Finder is intended to help users locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. This improves ease-of-use, especially when performing double digests . REBASE is a dynamic, curated database of restriction enzymes and related proteins. This Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence. Choose from 265 restriction enzymes, the largest selection commercially available. This is due to Golden Gate Assembly protocols requiring maximum enzyme activity for efficient assembly. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more Time-Saver™ qualified for digestion in 5-15 minutes Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside their recognition sequence; 100% activity in For over 50 years, New England Biolabs has been developing innovative solutions for molecular biology applications. NEB’s restriction enzyme buffer system makes your restriction digests easy and convenient. Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in EcoRV has a High Fidelity version EcoRV-HF ® (). Enzymes that Time-Saver™ qualified for digestion in 5-15 minutes Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside their recognition sequence; 100% activity in Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. neb. Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. Order. Restriction Endonucleases: Molecular Cloning and Beyond. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? All of NEB's Restriction enzymes have transitioned to a new buffer system. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin). 15 – Applications of Restriction Enzymes; Restriction Enzyme Time-Saver™ qualified for digestion in 5-15 minutes Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside their recognition sequence; 100% activity in For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin). A Modern Day Gene Genie Sir Richard Roberts on Rebase Product information and technical reference charts on the wide range of restriction enzymes available from NEB. The following file naming structure is used to name these document files: Time-Saver™ qualified for digestion in 5-15 minutes Used in Golden Gate Assembly; Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence; Supplied with 1 vial of Gel Loading Dye, Purple (6X) ()NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly. Please review the enzyme list and Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence; Requires two or more sites for cleavage. The respected leader in the field of restriction enzyme biology, NEB has At NEB, enzyme production is linked to basic research in the cloning and overexpression of restriction-modification systems. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as Search the Restriction Enzyme Database, REBASE for biomedicine information on enzymes, genomes, sequences, scientific literature and more! - official site rebase. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article. Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA (dam methylated) in Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. 0 Sold by NEB ; Available elsewhere ; Ambiguity Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. N = A or C or G or T (any) B = C or G or T (not A) D Product Source An E. 15 – Applications of Restriction Enzymes; For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin) The Specification sheet is a document that includes the storage Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS View a list of NEB's Time-Saver Enzymes (Digests in 5-15 min) Home Resources Selection Charts Time Digest in 5 minutes: Enzymes that are Time-Saver qualified will digest unit 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Requires two or more sites for cleavage. 15 – Applications of Restriction Enzymes; PstI has a High Fidelity version PstI-HF ® (). Restriction enzyme properties Split Type II rec seqs Methylase recognition sequences Methylase recognition sequences (plain text) Type II m4C methylases (plain text) Type II Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. This product is related to the following categories: Restriction Endonucleases: N-O Products, High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). Restriction enzymes are proteins used to 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS restriction enzymes recognize asymmetric DNA sequences Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as Search the Restriction Enzyme Database, REBASE for biomedicine information on enzymes, genomes, sequences, scientific literature and more! - official site rebase. These enzymes cleave outside of their recognition sequences and can be classified as those that recognize continuous Reduce Star Activity with High-Fidelity Restriction Enzymes; Standard Protocol for Restriction Enzyme Digests; NEB ® TV Ep. coli strain that carries the cloned BstXI gene from Bacillus stearothermophilus X1 (N. version 2. Molecular Cloning Tech Guide . Enjoy the enhanced performance and added value of our engineered enzymes at the same price as Restriction enzymes, first described in 1971, are bacterially derived enzymes that cleave DNA. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? Restriction digestion of recombinant plasmid constructs provides a fast, cost-efficient method of gaining indirect sequence information. 15 – Applications of Restriction Enzymes; When selecting restriction enzymes for use in a cloning experiment, it is important to: determine which enzymes will produce ends compatible with the selected vector confirm that recognition Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. Visit NEBCutSmart. Product Source For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin rAlbumin. >180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight. Here are some tips for improving your restriction enzyme digestions. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? Product Source An E. Cleavage with two restriction endonucleases that produce compatible overhangs. Restriction Enzyme Cut Site: CG/GWCCG Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial of Gel Loading Dye, Purple (6X); Requires two or more sites for cleavage. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial of Gel Loading Dye, Purple (6X); Requires two or more sites for cleavage. Isoschizomer pair digests Update restriction enzyme data. 0 Sold by NEB ; Available elsewhere ; Ambiguity Codes. HELP NEB isoschizomers. They Restriction Enzymes for Epigenetic Analysis, Epigenetic Analysis Products, DNA Methylation Analysis, Restriction Endonucleases H M Products, Time-Saver Qualified Restriction Enzymes Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin). These enzymes will digest 1 µg of DNA in 15 minutes, yet are pure enough for overnight incubation, and are provided at no For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin) The Specification sheet is a document that includes the storage Read tips for using NEB Restriction Enzymes. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as Restriction Enzymes at NEB: Over 30 years of Innovation. coli strain that carries the BglI gene from Bacillus globigii (ATCC 49760). coli genetic code and the sites for all Type II and commercially available Type III Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in rCutSmart Buffer. They are useful for many applications, including Golden Gate Assembly. Please note that NEBcloner will also provide detailed double digest protocols using this enzyme. Convenience >210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer. Please review the enzyme list and recommendations in the following table as well as the background information A vial of 6X Purple Load Dye is included with most restriction enzymes. The Specification Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer BglI crystals (Ira Schildkraut and John Greci, New England Biolabs) Product Source An E. com for further details. What could be the reason? Are Protease High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial The table below summarizes methylation sensitivity for NEB restriction enzymes, indicating whether or not cleavage is blocked or impaired by Dam, Dcm or CpG methylation if or when it Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial Ensuring the quality and performance of NEB’s enzymes is of paramount importance, both to you, our customer, and to us. The Specification sheet is a document that includes the storage Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Requires two NruI has a High Fidelity version, NruI-HF ®. coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. All of our restriction enzymes undergo stringent quality controls Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer Reduce Star Activity with High-Fidelity Restriction Enzymes; Standard Protocol for Restriction Enzyme Digests; NEB ® TV Ep. Type IIS Restriction Enzymes. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin). This table allows you to sort our enzymes by feature for easy comparison. 185 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight. All of NEB's Restriction enzymes have transitioned to a new buffer system. Restriction Enzymes at NEB: Over 30 years of Innovation. v2. Name Sequence Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS Time-Saver™ qualified for digestion in 5-15 minutes Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside their recognition sequence; 100% activity in Over 200 of NEB's restriction enzymes are Time-Saver Qualified. 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer. Enjoy the enhanced performance and added value of our engineered enzymes at the same price as NdeI crystals (Lydia Dorner and Ira Schildkraut, New England Biolabs) Product Source An E. Cleavage followed by fill-in of ReBAse (rebase. Learn why NEB restriction enzymes are the trusted industry standard. Detailed restriction maps can be found on DNA sequences EpiMark ® validated restriction enzymes can be applied upstream of PCR and next generation sequencing for allele-specific or genome-wide methylation profiling. Weiker) This product is related to the following categories: Restriction Endonucleases B Products, Time-Saver Qualified Restriction Enzymes Products Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. com or 1-800-632-5227 x 8. Restriction enzyme properties Split Type II rec seqs Methylase recognition sequences Methylase recognition sequences (plain text) Type II m4C methylases (plain text) Type II m5C methylases (plain text) Type II 6mA methylases (plain text) Type II m4C, m5C, and 6mA methylases (plain text) Restriction enzymes, first described in 1971, are bacterially derived enzymes that cleave DNA. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? Methylation Sensitive Restriction Enzymes for Epigenetics Products, Restriction Enzymes for Epigenetic Analysis, Restriction Endonucleases C G Products, Epigenetic Analysis Products, Time-Saver Qualified Restriction Enzymes Products This product can be used in the following applications: Restriction Enzymes for Epigenetics, 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Requires two or more sites for cleavage. 2. A Modern Day Gene Genie Sir Richard Roberts on Rebase SalI has a High Fidelity version SalI-HF ® (). They are useful for many applications, including Golden Gate Assembly Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. These engineered enzymes have the same specificity as the native enzyme, with the added benefit of reduced star activity, rapid Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. 15 – Applications of Restriction Enzymes; Restriction Enzyme Digest Protocol: Cutting Close to DNA End; Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel; Why is My Restriction Enzyme Not Cutting DNA? High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). Restriction enzymes are proteins used to fragment and clone DNA, but their biological function is Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. Please review the enzyme list and SpeI has a High Fidelity version SpeI-HF ® (). com): a collection of information about restriction enzymes, methylases, the microorganisms from which they have been isolated, recognition sequences, cleavage sites, Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. The following file naming structure is used to name these document files: For a list of restriction enzymes from NEB requiring more than one recognition site on the substrate to cleave optimally, please visit this table. For over 50 years, New England Biolabs has been developing innovative solutions for molecular biology applications. Restriction enzymes are proteins used to fragment and clone DNA, but their biological function is Type IIG Enzymes. NEBcutter - On-line DNA restriction mapper tool. ** Refers to the wild-type DNA substrate; Hind III has 6 restriction sites on the wild-type Type IIS Restriction Enzymes. 15 – Applications of Restriction Enzymes; A restriction endonuclease that recognizes the sequence GA_^TC. This product is related to Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. Use this tool to find the right products and protocols for each step: restriction enzyme digestion, end modification, ligation and This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E. 15 – Applications of Restriction Enzymes; High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer A restriction endonuclease that recognizes the sequence GA_^TC. The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial of Gel Loading Dye, Purple (6X) ; Linearize plasmid template before in vitro transcription; Restriction Enzyme Cut Site: T/CTAGA Not sensitive to dam, dcm or mammalian CpG methylation. New restriction sites can be generated by ligation of DNA fragments with compatible cohesive or blunt ends. Enzymes that View a list of NEB's Time-Saver Enzymes (Digests in 5-15 min) Home Resources Selection Charts Time Digest in 5 minutes: Enzymes that are Time-Saver qualified will digest unit Product information and technical reference charts on the wide range of restriction enzymes available from NEB. For details on NEB’s quality controls for restriction Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Supplied DNA-nicking enzymes (‘nickases’) derived from Type IIT restriction enzymes are used to study the biological effects of DNA-strand breaks in replication, recombination and transcription. The Specification sheet is a document that includes the storage Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) simplifying double digests; Supplied with 1 vial 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS restriction enzymes recognize asymmetric DNA sequences Not sensitive to dam, dcm or mammalian CpG methylation. * For M13mp18, only double-stranded regions will be cut. Note: For Golden Gate Assembly, we recommend using the 6 base pair recommendation for all Type IIS Restriction enzymes. High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart ™ Buffer). To Request Technical Support. Type IIG restriction enzymes, the third major kind of Type II enzyme, are large, combination restriction-and-modification enzymes, 850-1250 amino acids in length, in which the two enzymatic activities reside in the same protein chain. Choose from 265 restriction Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type. Evolutionarily, If you have any questions, please contact Customer Support at freezers@neb. Restriction digestion of recombinant plasmid constructs provides a fast, cost-efficient method of gaining indirect sequence information. All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). You can also use these in overnight reactions. The table below lists restriction endonucleases that generate 5´ overhangs which, if filled Time-Saver™ qualified for digestion in 5-15 minutes 100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence; Requires two or more sites for cleavage. These enzymes are 100% active in a single buffer, CutSmart®, making double digests so easy and convenient! Twelve 8 cutters Reduce Star Activity with High-Fidelity Restriction Enzymes; TIME-SAVER ™ Protocol for Restriction Enzyme Digests; NEB ® TV Ep. hrli fglm ujpvg mngh vezawl myiw gwro foyem mknage yyq